Annexin V-TAMRA Apoptosis Detection Reagent
Croyez fluorescent dye (TAMRA) conjugated Annexin V is highly purified product. During early apoptosis, cells will translocate membrane phosphatidylserine (PS) from the inner face of the membrane to the cell surface. The product can be used in one-step staining procedure without wash step within 20 minutes.
Component:
Storage:
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
Manual:
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-TAMRA and gently mix the cells and incubate for 15-20 minutes at RT in the dark.
*Option: Other reagents like 7-AAD or PI could be used in this step.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for rhodamine
(TRITC, corresponding to Annexin V-TAMRA).
Shipping Conditions:
Blue ice
| C08005-K01 | C08005-K02 | C08005-K03 | |
| Annexin V-TAMRA | 1 x 0.125 mL | 1 x 0.25 mL | 1 x 0.5 mL |
| 10X Binding Buffer | 1 x 2 mL | 2 x 2 mL | 3 x 2 mL |
Storage:
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
Manual:
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-TAMRA and gently mix the cells and incubate for 15-20 minutes at RT in the dark.
*Option: Other reagents like 7-AAD or PI could be used in this step.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for rhodamine
(TRITC, corresponding to Annexin V-TAMRA).
Shipping Conditions:
Blue ice